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The readings can also be conducted before the culture is evaluated for endpoint 자가지방이식 characterizations, like electrophysiology or airway marker expression.
There are various commercially out there resources for Most important airway epithelial cells. We have examined HAECs from two vendors, Lonza and Epithelix — both of those have resulted in prosperous ALI cultures.
Here is a list of revealed literature that cites culturing of nasal epithelial cells applying PneumaCult™ tradition media:
Just in case cell clumping is noticed right after thawing cryopreserved cells, it is suggested to filter aggregated suspensions by way of a 37 µm cell strainer (e.g. Catalog #27250) for ideal final results. In this case, the run time around the CellPore™ Transfection System could must be improved to ten seconds.
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Usually, HSAECs 지방이식 cultured in PneumaCult™-ALI-S Medium will form a totally differentiated cuboidal epithelium immediately after four to five months of tradition. Some donor variability could be expected.
Use cell counts to independent the sample into two tubes – “unstained” and “with viability dye”.
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